HSCCC Separations of Rutin Esters Obtained by Enzymatic Reaction Catalyzed by Lipase

The flavonoid rutin presents several pharmacological effects, despite this, its application in the pharmaceutical industry can be significantly limited by low bioavailability. development of lipophilic derivatives esterification hydroxyl groups with fatty acid chains may an effective strategy to change their physicochemical properties. This work aims use high-speed countercurrent chromatography (HSCCC) isolate esters produced reactions catalyzed immobilized lipase from Candida antarctica (Novozyme 435®). lipase-catalyzed synthesis (R3-R18:2) 2-methyl-2-butanol exhibited conversions that ranged 16 40% and highest conversion for short chain acids (R4-R12). After initial partitioning tests reaction mixture different solvent proportions followed thin layer (TLC) analysis, biphasic systems consisting HEMWat (hexane/ethyl acetate/methanol/water) were chosen separate esters. These separated first time via HSCCC. technique proved more advantageous than traditionally one since it allowed quick isolation high purity (≥ 90%) products. It also permitted a substrate recovery reuse other enzymatic reactions. Furthermore, results add valuable information, specially concerning structures elucidation not previously described.